More than 4730 compounds belong to the group of PFAS (per- and polyfluoroalkyl substances). Produced since the 1940s these compounds do not originate in nature, they are "forever chemicals" that are very persistent in the environment and in the human body. For reliable PFAS analysis MN have developed products that are ideally suited for sample preparation, sample integrity and determination of these harmful substances by HPLC.
The special phase for sample preparation – CHROMABOND® PFAS
CHROMABOND® PFAS is a polymer-based combination phase which contains a weak anion exchange functionality. The combination of different SPE phases makes it possible to use various interactions (dipole-dipole, ionic, hydrophobic, H-bond).
SPE product solutions – CHROMABOND® HR-X and HR-XAW
According to DIN 38407-42, EPA 537.1 and 533 guidelines, MACHEREY-NAGEL also offers further SPE product solutions for the enrichment of PFAS.
- CHROMABOND® HR-X: hydrophobic PS / DVB copolymer
- CHROMABOND® HR-XAW: weak mixed mode anion exchanger (WAX) PS / DVB copolymer
These allow outstanding recovery rates and high reproducibility.
PFAS in food using QuEChERS extraction and clean-up method
QuEChERS ("Quick, Easy, Cheap, Effective, Rugged and Safe") sample preparation products from MACHEREY-NAGEL ensure a time efficient and simple extraction of PFAS from food and a subsequent solid phase extraction for further sample clean-up according to procedure C-010.01 developed by the US Food and Drug Administration (FDA) for the measurement of 16 PFAS in food.
Plastic vials and fluorine-free closures for PFAS analysis
When you are doing PFAS analysis, it is crucial to select the right vials and closures for this application. Adsorption effects of glass as well as possible contaminations of the sample by particles from the septa, especially from the PTFE lamination, may put your analysis results at risk. MACHEREY-NAGEL offers polypropylene screw neck vials N 9 and snap ring vials N 11 as well as appropiate closures with a silicone/polyimide septum that – in contrast to PTFE laminated liners – is fluorine-free.
The special HPLC columns: NUCLEODUR® PFAS and NUCLEODUR® PFAS Delay
NUCLEODUR® PFAS HPLC columns are a perfect choice for analyzing PFAS substances. These columns show a high batch-to-batch reproducibility, are specially batch tested for PFAS analyses and are very well suited for LC-MS due to a low bleeding characteristics. The NUCLEODUR® PFAS Delay column provide high retention for PFAS compounds and are used to retain PFAS contaminants from the HPLC system, which could otherwise falsify the sample to be analyzed. For this purpose, the NUCLEODUR® PFAS Delay column is connected in flow direction between the mixing vessel and the sample injector.
Product selection according to ISO 21675:2019, DIN 38407-42, EPA 537.1, 533, 8327 and FDA C-010.01 guidelines
Product
Description | REF | PACK OF | ROWE
CODE |
| SPE columns dSPE centrifuge tubes | | | |
| CHROMABOND® PFAS, 85 μm, 6 mL / 300 mg | 730283 | 30 | VC0513 |
| CHROMABOND® HR-XAW, 85 μm, 6 mL / 500 mg | 730745 | 30 | VC0576 |
| CHROMABOND® HR-XAW, 85 μm, 3 mL / 60 mg | 730747 | 30 | VC0577 |
| CHROMABOND® HR-X, 45 μm, 3 mL / 200 mg | 730931P45 | 30 | VC0554 |
| CHROMABOND® HR-X, 85 μm, 6 mL / 500 mg | 730939 | 30 | VC0557 |
CHROMABOND® QuEChERS Mix XII, extraction mix consisting of:
4000 mg MgSO4, 1000 mg NaCl, individually weighed and filled into 15 mL centrifuge tubes (PP) with screw cap (PE) | 730648 | 50 | VC0449 |
CHROMABOND® QuEChERS Mix L Diamino / Carbon clean-up mix consisting of:
900 mg MgSO4, 300 mg CHROMABOND® Diamino, 150 mg CHROMABOND® Carbon, individually weighed and filled into 15 mL centrifuge tubes (PP) with screw cap (PE) | 7300008 | 50 | VC0578 |
CHROMABOND® QuEChERS Mix XX Diamino clean-up mix (acc. to AOAC) consisting of:
150 mg MgSO4, 50 mg CHROMABOND® Diamino individually weighed and filled into 2 mL centrifuge tubes (PP) with snap cap (PP) | 730670.2 | 50 | VC0579 |
| HPLC columns | - | - | |
| EC 100/2 NUCLEODUR® PFAS, 3 μm | 760666.20 | 1 | VC0544 |
| EC 50/2 NUCLEODUR® PFAS, 3 μm | 760663.20 | 1 | VC0543 |
| EC 50/2 NUCLEODUR® PFAS Delay, 5 μm | 760673.20 | 1 | VC0545 |
| Vials and caps | - | - | |
| 0.3 mL N 9 screw neck vial, PP transparent, 11.6 x 32.0 mm, inner cone | 702009 | 100 | VV0039 |
| 0.7 mL N 9 screw neck vial, PP transparent, 11.6 x 32.0 mm, round bottom insert | 702010 | 100 | VV0076 |
| 1.5 mL N 9 screw neck vial, PP transparent, 11.6 x 32.0 mm, flat bottom | 702500 | 100 | VV0077 |
| 0.3 mL N 9 screw neck vial, PP amber, 11.6 x 32.0 mm inner cone | 702172 | 100 | VV0075 |
| 0.3 mL N 11 snap ring/crimp neck vial PP transparent, 11.6 x 32.0 mm inner cone | 702809 | 100 | VV0097 |
| 0.7 mL N 11 snap ring/crimp neck vial, PP transparent, 11.6 x 32.0 mm round bottom inse | 702174 | 100 | VV0099 |
| 0.3 mL N 11 snap ring/crimp neck vial, PP amber, 11.6 x 32.0 mm inner cone | 702173 | 100 | VV0098 |
| N 9 screw closure, PP, blue, center hole, silicone white / polyimide orange, 1.0 mm | 702402 | 100 | VC0512 |
| N 11 snap ring closure, PE (soft), light blue, center hole, silicone white / polyimide orange, 1.0 mm | 702403 | 100 | VC0566 |
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03 Feb 2023
